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KMID : 1130320070500020151
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Korean Journal of Pediatrics
2007 Volume.50 No. 2 p.151 ~ p.156
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A study of serotyping of Streptococcus pneumoniae by multibead assay
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Cho Ky-Young
Lee Jung-Ah
Cho Sung-Eun
Kim Nam-Hee
Lee Jin-A
Hong Ki-Sook
Lee Hoan-Jong
Kim Kyung-Hyo
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Abstract
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Purpose: Streptococcus pneumoniae is a major etiologic agent for pneumonia, meningitis, otitis media, and sepsis among young children. Multi-drug resistant strains have raised great concern worldwide, thus the importance of prevention with vaccines has been emphasized. However, vaccines may force the appearance of pneumococcal infections by nonvaccine serotypes. Thus, distribution of pneumococcal serotypes should be monitored to estimate vaccine efficacy. We used a new and efficient multibead assay in determining pnemococcal serotypes.
Methods: From January to February 2005, 643 children were recruited from ten day care centers to isolate pneumococci from their oropharynx. Pneumococcal serotyping was performed on 62 pneumococcal isolates from 60 children by multibead assay. This immunoassay required two sets of latex particles coated with pneumococcal polysaccharides and serotype-specific antibodies. Twenty four newly developed monoclonal antibodies specific for common serotypes and a pool of polyclonal rabbit sera for some of the less common serotypes were used.
Results: The most prevalent pneumococcal serotypes were serotype 6A, 19A, 19F, 23F, and 11A/ D/F which accounted more than 50 precent of all the 62 pneumococcal isolates. We found that multibead assay can be performed very rapidly and objectively.
Conclusion: This multibead immunoassay was very useful in serotyping clinical isolates of S. pneumoniae because it was simple, reliable and fast.
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KEYWORD
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Streptococcus pneumoniae, serotyping, Immunoassay
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